Training

Operation of Krios G3 Cryo - TEM

The Titan Krios G3 cryo transmission electron microscope (cryo-TEM) is the most powerful high resolution electron microscope specializing in cryo-based 3D characterization of biological samples. It is equipped with:

  • A Schottky X-FEG high brightness electron source.
  • Cs image corrector for compensation of spherical aberration (Cs) and semi-automated correction of astigmatism [0.08 nm (TEM) information limit].
  • Flexible high tension range from 60 to 300 kV.
  • Symmetric constant power C-TWIN objective lens with wide pole piece gap of 11 mm.
  • Cryo autoloader for robotic loading of up to 12 frozen, hydrated samples.
  • Volta phase plate solution for tunable phase shifting to optimize contrast.
  • Low dose software kit to minimize electron dosage.
  • Semi-automated data collection for continuous multi-day data acquisition.
  • Falcon 3EC pre-GIF direct electron detector camera.
  • Gatan BioQuantum LS / 967 energy filter coupled to a Gatan K2 Summit direct electron detector camera.
  • Automatic apertures and reproducible recall.
  • Rotation-free imaging for clear orientation.
  • A computerized 5-axis specimen stage with piezo-enabled drift compensation.



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