Training

Operation of Krios G4 Cryo - TEM

This is a new generation of high-resolution electron microscope for high-speed single particles analysis, cellular tomography and micro electron diffraction (MicroED). It is equipped with:

  • A Schottky X-FEG high brightness electron source.
  • Flexible high-tension ranges from 60 to 300 kV with high-stability generator.
  • A five-lens condenser system with symmetric constant power C-TWIN objective lens, etc. for parallel illumination over a wide and variable field of view.
  • High-speed drift correction, Semi-automated data collection for continuous multi-day data acquisition.
  • Low dose software kit to minimize electron dosage.
  • Flu camera, Ceta-D camera 300 kV, Falcon 3EC and Falcon 4 camera 300 kV with DMP server.
  • Automatic apertures and reproducible recall.
  • Rotation-free imaging for clear orientation.
  • A computerized 4-axis specimen stage with piezo-enabled drift compensation, and ±70 degree alpha-tilt.
  • Cryo autoloader for robotic loading of up to 12 frozen, hydrated samples.
  • TEM tomography data acquisition software, Velox imaging software, linear distortion correction, MicroED package, etc.



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