Training

Operation of Aquilos 2 Cryo - FIB

This is a second-generation dedicated cryo-DualBeam focused ion beam microscope for preparing cryo-lamella samples for TEM tomography of frozen-hydrated biological materials. It is equipped with:

  • NICol UHR non-immersion field emission-SEM column and Sidewinder ion column.
  • In-lens detection system: segmented lower (T1) and upper detector (T2), Everhart-Thornley SE Detector (ETD), ion conversion and electron (ICE) detector for secondary ions and secondary electrons.
  • 110 x 110 mm eucentric stage, Fully rotatable cryo-stage (<-170°C at the sample), Tilt range at cryo (eucentric WD): -15° to +55°.
  • Automatic Aperture System.
  • CCD IR Camera, In-chamber Nav-Cam.
  • Cryo Kit, incl. Cryo transfer system, Cryo loading station, conductive deposition GIS, In-chamber retractable sputter coater, EasyLift nanomanipulator, Cryo-FIB AutoGrids.
  • Maps 3 software for S/TEM with correlative workflow, AutoScript DB academic, AutoSlice & View, AutoTEM, Cryo for milling automation, SPI™ and iSPI™ software, etc.


Please be aware that this course has the following pre-requisite (or equivalent experience): "Operation of Focused Ion Beam System (FEI Helios G3 DualBeam FIB/SEM)". Questions about your qualification to enroll in this course should be directed to Nan Yao, director of the Imaging and Analysis Center. Please do not enroll in this course unless you are certain you have met the pre-requisite requirements. Thank you!

Schedule

Dates
Times
Status
01/20/2026
9:30am - 12noon
CLOSED
02/17/2026
9:30am - 12noon
CLOSED
03/16/2026
9:30am - 12noon
04/16/2026
9:30am - 12noon
05/20/2026
9:30am - 12noon


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